Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:200–1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
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Reviews
IR238-892 anti-Cytokeratin 20 antibody WB image
Anti-Cytokeratin 20 antibody - at 1/2000 dilution
Lysates/proteins at 45 μg per lane.
This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR238-892 overnight at 4°C.
IR238-892 anti-Cytokeratin 20 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 20 with IR238-892 at 1/200.
IR238-892 anti-Cytokeratin 20 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 20 with IR238-892 at 1/200.
IR238-892 anti-Cytokeratin 20 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR238-892 anti-Cytokeratin 20 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR238-892 anti-Cytokeratin 20 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR238-892 anti-Cytokeratin 20 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.