Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100–1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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Reviews
IR236-890 anti-Cytokeratin 18/19 antibody WB image
Anti-Cytokeratin 18/19 antibody - at 1/2000 dilution. Lysates at 30 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR236-890 overnight at 4°C.
IR236-890 anti-Cytokeratin 18/19 antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR236-890 anti- Cytokeratin 18/19 antibody (red) at 1:150 and 4°C.
IR236-890 anti-Cytokeratin 18/19 antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR236-890 anti- Cytokeratin 18/19 antibody (red) at 1:150 and 4°C.
IR236-890 anti-Cytokeratin 18/19 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 18/19 with IR236-890 at 1/200.
IR236-890 anti-Cytokeratin 18/19 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 18/19 with IR236-890 at 1/200.