Product Includes
Application Dilution
Immunofluorescence 1:500–1:800
Immunohistochemistry (Paraffin) 1:400–1:600
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
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Reviews
IR229-871 anti-Cytokeratin 8 antibody WB image
Anti-Cytokeratin 8 antibody - at 1/2000 dilution
Lysates at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR229-871 overnight at 4°C.
IR229-871 anti-Cytokeratin 8 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with IR229-871 at 1/400.
IR229-871 anti-Cytokeratin 8 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with IR229-871 at 1/400.
IR229-871 anti-Cytokeratin 8 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR229-871 anti- Cytokeratin 8 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.