Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:200–1:500
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
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Reviews
IR228-868 anti-Cytokeratin 7 antibody IHC image
Side by side with VANTANA IVD grade antibody
IR228-868 anti-Cytokeratin 7 antibody WB image
Anti-Cytokeratin 7 antibody - at 1/2000 dilution. Lysates at 45 μg per lane. Nitrocellulose membrane was then blocked an hour before being incubated with IR228-868 overnight at 4°C.
IR228-868 anti-Cytokeratin 7 antibody_Colorectal carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling Cytokeratin 7 with IR228-868 at 1/300.
IR228-868 anti-Cytokeratin 7 antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with IR228-868 at 1/200.
IR228-868 anti-Cytokeratin 7 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR228-868 anti- Cytokeratin 7 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR228-868 anti-Cytokeratin 7 antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with IR228-868 at 1/200.
IR228-868 anti-Cytokeratin 7 antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with IR228-868 at 1/200.
IR228-868 anti-Cytokeratin 7 antibody_Breast carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling Cytokeratin 7 with IR228-868 at 1/300.
IR228-868 anti-Cytokeratin 7 antibody_Liver carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling Cytokeratin 7 with IR228-868 at 1/300.
IR228-868 anti-Cytokeratin 7 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR228-868 anti- Cytokeratin 7 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR228-868 anti-Cytokeratin 7 antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with IR228-868 at 1/200.
IR228-868 anti-Cytokeratin 7 antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with IR228-868 at 1/200.
IR228-868 anti-Cytokeratin 7 antibody_Lymph node carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling Cytokeratin 7 with IR228-868 at 1/300.