Product Includes
Application Dilution
Immunofluorescence 1:400 – 1:800
Immunohistochemistry (Paraffin) 1:400 – 1:800
Flow Cytometry 1:300 – 1:500
Storage
PBS (pH 7.4), 40% Glycerol. 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]
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Reviews
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : Pan-Cytokeratin antibody (IR222-861)
Data from : Nebulum Technologies Co., Ltd.
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : CTC (Circulating Tumor Cell)
Antibody : Pan-Cytokeratin antibody (IR222-861)
Data from : Nebulum Technologies Co., Ltd.
Technology : 3D bio-imaging
Customer feedback Image
Application : ICC/IF
Condition / Dilution ：1-400
Data from : CytoAurora Biotechnologies, Inc.
IR222-861 anti-pan Cytokeratin antibody IHC image
Side by side with VANTANA IVD grade antibody
IR222-861 anti-pan Cytokeratin antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with IR222-861 at 1/400.
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : CTC (Circulating Tumor Cell)
Antibody : Pan-Cytokeratin antibody (IR222-861)
Data from : Nebulum Technologies Co., Ltd.
Technology : 3D bio-imaging
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : Pan-Cytokeratin antibody (IR222-861)
Data from : Nebulum Technologies Co., Ltd.
IR222-861 anti-pan Cytokeratin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR222-861 anti-pan Cytokeratin antibody (green) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR222-861 anti-pan Cytokeratin antibody WB image
Anti-pan Cytokeratin antibody - at 1/3000 dilution
IR222-861 anti-pan Cytokeratin antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with IR222-861 at 1/400.
IR222-861 anti-pan Cytokeratin antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with IR222-861 at 1/400.
IR222-861 anti-pan Cytokeratin antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with IR222-861 at 1/400.
IR222-861 anti-pan Cytokeratin antibody IHC image
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with IR222-861 at 1/400.
IR222-861 anti-pan Cytokeratin antibody FACS image
Product：pan Cytokeratin antibody
Catalog：IR222-861
Cell : MCF-7
pan Cytokeratin (IR222-861) : 1-350
IR222-861 anti-pan Cytokeratin antibody WB image
Anti-pan Cytokeratin antibody - at 1/3000 dilution
Lysates at 45 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR222-861 overnight at 4°C
IR222-861 anti-pan Cytokeratin antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR222-861 anti-pan Cytokeratin0 antibody (red) at 1:300 and 4°C.
IR222-861 anti-pan Cytokeratin antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with IR222-861 at 1/400.