Product Includes
Application Dilution
Immunofluorescence 1:300–1:500
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
Reviews
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Application : Western blot
Condition / Dilution：1-1000
Sample : H1299, BT-549
Data from : China Medical University
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000
IR129-522 anti-CHOP antibody WB image
All lanes : Anti-CHOP antibody - at 1/500 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR129-522 overnight at 4°C.
IR129-522 anti-CHOP antibody WB image
All lanes : Anti-CHOP antibody - at 1/800 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR129-522 overnight at 4°C.
IR129-522 anti-CHOP antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR129-522 anti- CHOP antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR129-522 anti-CHOP antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CHOP with IR129-522 at 1/200.