Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1: 100-1:200
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
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Reviews
IR123-314 anti-p27 Kip1 antibody WB image
p27 Kip1 antibody at 1/500 dilution.
Lysates at 30 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR123-314 overnight at 4°C
IR123-314 anti-p27 Kip1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p27 Kip1 with IR123-314 at 1/150.