Product Includes
Application Dilution
Immunofluorescence 1:100-1:200
Immunohistochemistry (Paraffin) 1:100–1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. [provided by RefSeq, Dec 2019]
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Reviews
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000, 3%BSA
Sample : HT-29 drug treatment
Data from : Taipei Medical University
Customer feedback Image
Application : FFPE immunohistochemistry
Condition / Dilution：1-200
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Sample : human breast cancer
Data from : Kaohsiung Medical University
Customer feedback Image
Application : Western blot
Condition / Dilution： 1-1000
Sample : A172
Data from : Taipei Medical University
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000, 3%BSA
Sample : HT-29 drug treatment
Data from : Taipei Medical University
IR117-294 anti-Cyclin D1 antibody WB image
Anti-Cyclin D1 antibody at 1/1000 dilution
Lysates at 30 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR117-294 overnight at 4°C.
IR117-294 anti-Cyclin D1 antibody WB image
Anti-Cyclin D1 antibody at 1/1000 dilution
Lysates at 30 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR117-294 overnight at 4°C.
IR117-294 anti-Cyclin D1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cyclin D1 with IR117-294 at 1/200.
IR117-294 anti-Cyclin D1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cyclin D1 with IR117-294 at 1/200.
IR117-294 anti-Cyclin D1 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR117-294 anti- Cyclin D1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.